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蛋白质与碳水化合物的高温共轭作用。

High temperature conjugation of proteins with carbohydrates.

作者信息

Boratyński J, Roy R

机构信息

Department of Tumor Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.

出版信息

Glycoconj J. 1998 Feb;15(2):131-8. doi: 10.1023/a:1007067513242.

DOI:10.1023/a:1007067513242
PMID:9557872
Abstract

A new procedure was used to conjugate lactose and dextran with BSA without using coupling or activating reagents. The method is simple, rapid and cheap. Reducing sugars covalently bind to proteins when lyophilized together and briefly heated to a high temperature.

摘要

一种新方法被用于在不使用偶联剂或活化剂的情况下将乳糖和葡聚糖与牛血清白蛋白（BSA）偶联。该方法简单、快速且成本低廉。还原糖在冻干并短暂加热至高温时会与蛋白质共价结合。

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本文引用的文献

1
THYMIDINE DIPHOSPHATE 4-ACETAMIDO-4,6-DIDEOXYHEXOSES. II. PURIFICATION AND PROPERTIES OF THYMIDINE DIPHOSPHATE D-GLUCOSE OXIDOREDUCTASE.
J Biol Chem. 1965 Mar;240:1305-8.
2
Thymidine diphosphate 4-keto-6-deoxy-d-glucose, an intermediate in thymidine diphosphate L-rhamnose synthesis in Escherichia coli strains.胸苷二磷酸4-酮-6-脱氧-D-葡萄糖，大肠杆菌菌株中胸苷二磷酸L-鼠李糖合成的中间体。
J Biol Chem. 1962 Oct;237:3014-26.
3
The enzymatic synthesis of thymidine-linked sugars. II. Thymidine diphosphate L-rhamnose.胸腺嘧啶核苷连接糖的酶促合成。II. 胸腺嘧啶核苷二磷酸L-鼠李糖。
用微核试验测定的晚期糖基化终产物（MAGE）的遗传毒性和促凋亡活性可被其低相对分子质量的对应物所抑制。
Genes (Basel). 2021 May 13;12(5):729. doi: 10.3390/genes12050729.
4
Glycosylation of Membrane Proteins Using -Glycosyltransferase.使用α-糖基转移酶对膜蛋白进行糖基化修饰。
ACS Omega. 2021 Apr 26;6(18):12133-12142. doi: 10.1021/acsomega.1c00835. eCollection 2021 May 11.
5
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6
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Biosci Rep. 2016 Oct 14;36(5). doi: 10.1042/BSR20160233. Print 2016 Oct.
7
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4
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5
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9
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J Biol Chem. 1965 Nov;240(11):4128-32.
10
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Adv Carbohydr Chem Biochem. 1966;21:143-207. doi: 10.1016/s0096-5332(08)60317-3.