在碱性条件下美拉德反应的氨基脱氧酮糖产物转化为Nε-（羧甲基）赖氨酸。
Conversion of Amadori product of Maillard reaction to Nepsilon-(carboxymethyl)lysine in alkaline condition.
作者信息
Nagai R, Ikeda K, Kawasaki Y, Sano H, Yoshida M, Araki T, Ueda S, Horiuchi S
机构信息
Department of Biochemistry, Kumamoto University School of Medicine, Honjo, Japan.
出版信息
FEBS Lett. 1998 Mar 27;425(2):355-60. doi: 10.1016/s0014-5793(98)00263-4.
Nepsilon-(carboxymethyl)lysine (CML) is known to be formed by oxidative cleavage of Amadori products between C-2 and C-3 of the carbohydrate chain. We report here that CML formation from Amadori compounds is highly accelerated under alkaline conditions. Incubation of glycated human serum albumin (HSA) in 0.1 N NaOH led to the formation of CML whereas glycated HSA reduced by NaCNBH3 or non-glycated HSA did not generate CML. Nalpha-t-butyloxycarbonyl-Nepsilon-fructoselysine (Boc-FL), a model compound of Amadori product, was converted to CML under alkaline conditions. CML level of human sera (n=224) preincubated with 0.1 N NaOH correlated well with glycated albumin value (r=0.912) and hemoglobin A1c (r=0.797).
Nε-（羧甲基）赖氨酸（CML）已知是由碳水化合物链C-2和C-3之间的阿马多里产物氧化裂解形成的。我们在此报告，在碱性条件下，由阿马多里化合物形成CML的过程会大大加速。将糖化人血清白蛋白（HSA）在0.1 N NaOH中孵育会导致CML的形成，而经NaCNBH3还原的糖化HSA或未糖化的HSA则不会产生CML。阿马多里产物的模型化合物Nα-叔丁氧羰基-Nε-果糖赖氨酸（Boc-FL）在碱性条件下会转化为CML。用0.1 N NaOH预孵育的人血清（n = 224）的CML水平与糖化白蛋白值（r = 0.912）和血红蛋白A1c（r = 0.797）密切相关。
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