利用同步辐射红外显微光谱法对活细胞进行高分辨率化学成像。
Highly resolved chemical imaging of living cells by using synchrotron infrared microspectrometry.
作者信息
Jamin N, Dumas P, Moncuit J, Fridman W H, Teillaud J L, Carr G L, Williams G P
机构信息
Commissariat à l'Energie Atomique-Institut National des Sciences et Techniques Nucléaires, F91191 Gif Sur Yvette Cedex, France.
出版信息
Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):4837-40. doi: 10.1073/pnas.95.9.4837.
Abstract
Using synchrotron radiation as an ultra-bright infrared source, we have been able to map the distributions of functional groups such as proteins, lipids, and nucleic acids inside a single living cell with a spatial resolution of a few microns. In particular, we have mapped the changes in the lipid and protein distributions in both the final stages of cell division and also during necrosis.
摘要
利用同步辐射作为超亮红外光源，我们得以在空间分辨率达到几微米的情况下，绘制单个活细胞内蛋白质、脂质和核酸等官能团的分布图谱。特别是，我们已经绘制出了细胞分裂末期以及坏死过程中脂质和蛋白质分布的变化情况。
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