Fujita M, Yamamoto M
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Japan.
Curr Genet. 1998 Apr;33(4):248-54. doi: 10.1007/s002940050333.
The Schizosaccharomyces pombe sck2 gene, originally identified as SPAC22E12.14c in the genome-sequencing project, encodes a putative protein kinase highly similar to Saccharomyces cerevisiae Sch9p and S. pombe Sck1p, both of which can suppress loss of cAMP-dependent protein kinase (PKA) if over-produced. Over-expression of sck2 suppressed typical phenotypes of PKA-defective cells, including ectopic mating, slow growth and short cell morphology. Wild-type cells over-expressing sck2 behaved like the PKA-hyperactive mutant. Disruption of sck2 caused no obvious phenotype, but it intensified de-repression for sexual development when combined with the disruption of sck1. The pka1 sck1 sck2 triple disruptant could grow but only very slowly. Whereas disruption of sck1 enhanced the inefficiency of Deltapka1 spores in germination, disruption of sck2 did not. These results suggest that the molecular function of Sck2p largely overlaps with that of Sck1p, but also that they differ somewhat either quantitatively or qualitatively.
粟酒裂殖酵母的sck2基因最初在基因组测序项目中被鉴定为SPAC22E12.14c，它编码一种假定的蛋白激酶，与酿酒酵母的Sch9p和粟酒裂殖酵母的Sck1p高度相似，后两者如果过量表达都能抑制依赖cAMP的蛋白激酶（PKA）的缺失。sck2的过表达抑制了PKA缺陷细胞的典型表型，包括异位交配、生长缓慢和细胞形态短小。过表达sck2的野生型细胞表现得像PKA过度活跃的突变体。sck2的缺失没有导致明显的表型，但当与sck1的缺失相结合时，它会加剧对性发育的去抑制作用。pka1 sck1 sck2三重缺失突变体能够生长，但非常缓慢。虽然sck1的缺失增强了Δpka1孢子萌发的低效性，但sck2的缺失却没有。这些结果表明，Sck2p的分子功能在很大程度上与Sck1p重叠，但在数量或质量上也存在一些差异。
