Levin D E, Bishop J M
Department of Microbiology and Immunology, University of California, San Francisco 94143.
Proc Natl Acad Sci U S A. 1990 Nov;87(21):8272-6. doi: 10.1073/pnas.87.21.8272.
Mixed synthetic oligonucleotides encoding a sequence conserved among tyrosine-specific protein kinases were used to probe the genome of the fission yeast Schizosaccharomyces pombe. A single gene (kin1+) was isolated that encodes a putative protein kinase closely related to the KIN1- and KIN2-encoded serine/threonine-specific protein kinases of Saccharomyces cerevisiae. kin1+ is transcribed into a 3.5-kilobase mRNA that contains an uninterrupted open reading frame encoding a polypeptide of 98 kDa. In contrast to results obtained with kin mutants of S. cerevisiae, disruption of the Sc. pombe kin1+ gene resulted in recessive morphological and growth defects. kin1-disrupted cells grew slowly on enriched medium and grew as spheres, in contrast to wild-type Sc. pombe cells, which grow as rods. Relative to kin1+ cells, kin1-disrupted cells were differentially sensitive to lysis by treatment with alpha- and beta-glucanases, suggesting an alteration in either the composition or the organization of their cell walls.
编码酪氨酸特异性蛋白激酶中保守序列的混合合成寡核苷酸被用于探测裂殖酵母粟酒裂殖酵母的基因组。分离出了一个单一基因（kin1+），它编码一种假定的蛋白激酶，与酿酒酵母中由KIN1和KIN2编码的丝氨酸/苏氨酸特异性蛋白激酶密切相关。kin1+转录成一个3.5千碱基的mRNA，其中包含一个不间断的开放阅读框，编码一个98 kDa的多肽。与酿酒酵母kin突变体的结果相反，粟酒裂殖酵母kin1+基因的破坏导致隐性形态和生长缺陷。与野生型粟酒裂殖酵母细胞（呈杆状生长）相比，kin1基因被破坏的细胞在富集培养基上生长缓慢，呈球形生长。相对于kin1+细胞，kin1基因被破坏的细胞对用α-和β-葡聚糖酶处理后的裂解具有不同的敏感性，这表明它们细胞壁的组成或组织发生了改变。
