一种针对海豚淋巴细胞的单克隆抗体（6E9），它能识别牛的主要组织相容性复合体II类抗原。

A monoclonal antibody against dolphin lymphocytes (6E9) which recognizes bovine MHC class II antigens.

作者信息

Shirai K, Sakai T, Fukuda M, Oike T

机构信息

Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, Nihon University, Kanagawa, Japan.

出版信息

J Vet Med Sci. 1998 Mar;60(3):291-3. doi: 10.1292/jvms.60.291.

DOI:10.1292/jvms.60.291

PMID:9560774

Abstract

A monoclonal antibody, 6E9 established from mice injected with dolphin peripheral blood lymphocytes (PBLs) was characterized. In addition to its reactivity against 89.4% of dolphin PBLs, 6E9 reacted with 33.1% of bovine PBLs of which 22% were CD5+, 11.1% were CD5-. 6E9 recognized a 34 kD protein on the surface of dolphin and bovine PBLs. Analysis of the protein's N-terminal amino acid sequence indicated that 6E9 recognizes bovine major histocompatibility complex (MHC) class II antigens. These results suggested that 6E9 recognized MHC class II antigens on bovine PBLs. As we have already produced an anti-dolphin MHC class I monoclonal antibody, analysis of immune system using these monoclonal antibodies will advance our understanding of the evolution of the mammalian immune system.

摘要

对一种从注射了海豚外周血淋巴细胞（PBL）的小鼠体内产生的单克隆抗体6E9进行了特性分析。除了与89.4%的海豚PBL发生反应外，6E9还与33.1%的牛PBL发生反应，其中22%为CD5阳性，11.1%为CD5阴性。6E9识别出海豚和牛PBL表面的一种34 kD蛋白。对该蛋白N端氨基酸序列的分析表明，6E9识别牛主要组织相容性复合体（MHC）II类抗原。这些结果表明，6E9识别牛PBL上的MHC II类抗原。由于我们已经制备了一种抗海豚MHC I类单克隆抗体，利用这些单克隆抗体对免疫系统进行分析将有助于我们深入了解哺乳动物免疫系统的进化。