一种使用单克隆抗体纯化抗原的斑点酶联免疫吸附试验，用于诊断由异盘并殖吸虫引起的并殖吸虫病。

A dot-ELISA test using monoclonal antibody-purified antigens for the diagnosis of paragonimiasis caused by Paragonimus heterotremus.

作者信息

Maleewong W, Intapan P M, Wongkham C, Pajongthanasaris M, Morakote N, Tapchaisri P, Chaicumpa W

机构信息

Department of Parasitology, Faculty of Medicine, Khon Kaen University, Thailand.

出版信息

Southeast Asian J Trop Med Public Health. 1997 Sep;28(3):621-3.

PMID:9561619

Abstract

A dot enzyme-linked immunosorbent assay (dot-ELISA) using antigens purified by monoclonal antibody-affinity chromatography was developed for detecting antibodies to Paragonimus heterotremus in four groups of subjects. They consisted of 30 patients with P. heterotremus infection, 93 patients with other parasitic infections, 18 patients with pulmonary tuberculosis and 30 normal, healthy controls. Sensitivity, specificity, as well as positive and negative predictive values of the test were 100, 97, 88, and 100%, respectively.

摘要

采用经单克隆抗体亲和层析纯化的抗原建立了斑点酶联免疫吸附试验（dot-ELISA），用于检测四组受试者体内的异盘并殖吸虫抗体。这四组受试者包括30例异盘并殖吸虫感染者、93例其他寄生虫感染者、18例肺结核患者以及30名正常健康对照者。该检测方法的敏感性、特异性、阳性预测值和阴性预测值分别为100%、97%、88%和100%。