Maleewong W, Intapan P M, Wongkham C, Wongratanacheewin S, Tapchaisri P, Morakote N, Chaicumpa W
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Thailand.
J Parasitol. 1997 Dec;83(6):1075-8.
An antigen capture enzyme-linked immunosorbent assay (antigen capture-ELISA) and DNA hybridization technique were developed and evaluated for their application in the detection of Paragonimus heterotremus infection in experimentally infected cats. An IgG fraction prepared from serum of a rabbit immunized with P. heterotremus excretory-secretory (ES) products was used as the capture antibody. An IgG1 monoclonal antibody specific to the 22- and 31.5-kDa ES products of P. heterotremus was used as the antigen probe. As little as 0.24 ng of the ES products could be detected by this technique. A specific P. heterotremus DNA probe derived from the P. heterotremus genomic DNA library containing 1,500 base pairs was used in a dot-blot hybridization assay for the detection of parasite DNA. The radioactively labeled probe could detect DNA released from as few as 2 P. heterotremus eggs. Both ELISA and DNA hybridization were found to have 100% specificity, with sensitivities of 73.7% and 100%, respectively.
开发并评估了一种抗原捕获酶联免疫吸附测定法（抗原捕获酶联免疫吸附测定，antigen capture-ELISA）和DNA杂交技术，用于检测实验感染猫的异盘并殖吸虫感染。用异盘并殖吸虫排泄-分泌（ES）产物免疫的兔血清制备的IgG组分用作捕获抗体。对异盘并殖吸虫22 kDa和31.5 kDa ES产物具有特异性的IgG1单克隆抗体用作抗原探针。该技术可检测低至0.24 ng的ES产物。使用从包含1500个碱基对的异盘并殖吸虫基因组DNA文库中获得的特异性异盘并殖吸虫DNA探针，进行斑点印迹杂交测定以检测寄生虫DNA。放射性标记的探针可检测低至2个异盘并殖吸虫卵释放的DNA。发现ELISA和DNA杂交的特异性均为100%，灵敏度分别为73.7%和100%。
