Eamsobhana P, Yong H S, Mak J W, Wattanakulpanich D
Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bankok, Thailand.
Southeast Asian J Trop Med Public Health. 1997 Sep;28(3):624-8.
A dot-blot ELISA was compared with a previously performed sandwich ELISA for the detection of Parastrongylus cantonensis antigens in sera from patients. Using the same monoclonal antibody and the same sera, 6 of 10 sera (60%) from parastronglyiasis patients were positive in dot-blot ELISA, whereas with sandwich ELISA, 5 of the same patient sera (50%) were positive. The specificity in both assays was 100% using 50 sera from patients with other parasitic diseases; of these, 10 each were from patients with cysticercosis, filariasis, gnathostomiasis, malaria and toxocariasis. The control group consisted of 53 sera from normal health Thais and Malaysians. The sensitivity of the assays was, however, slightly better with dot-blot ELISA and because it is simple, quick and cost-effective, it may be a test of choice for specific diagnosis of human parastrongyliasis.
将斑点印迹酶联免疫吸附测定法（dot-blot ELISA）与先前进行的夹心酶联免疫吸附测定法（sandwich ELISA）进行比较，以检测患者血清中的广州管圆线虫抗原。使用相同的单克隆抗体和相同的血清，广州管圆线虫病患者的10份血清中有6份（60%）在斑点印迹酶联免疫吸附测定法中呈阳性，而在夹心酶联免疫吸附测定法中，相同患者的5份血清（50%）呈阳性。使用来自其他寄生虫病患者的50份血清时，两种测定法的特异性均为100%；其中，分别有10份血清来自囊尾蚴病、丝虫病、颚口线虫病、疟疾和弓蛔虫病患者。对照组由53份来自健康泰国人和马来西亚人的血清组成。然而，斑点印迹酶联免疫吸附测定法的检测灵敏度略高，并且由于其操作简单、快速且具有成本效益，它可能是人类广州管圆线虫病特异性诊断的首选检测方法。
