Brink J, Sherman M B, Berriman J, Chiu W
Verna and Marrs McLean Department of Biochemistry, National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, TX 77030, USA.
Ultramicroscopy. 1998 Apr;72(1-2):41-52. doi: 10.1016/s0304-3991(97)00126-5.
We describe procedures to assess charging of biological specimens under electron irradiation in an electron cryomicroscope. Charging can be observed by an expansion of the illuminating beam, blurring of electron diffraction patterns and by beam "footprints" on the specimen. Discharging can also be seen in the defocused electron diffraction mode. We investigated the influence of a variety of factors on the magnitude and visibility of charging. A reduction of charging is noticed when part of the adjacent carbon film is included in the irradiated specimen area.
我们描述了在电子冷冻显微镜中评估生物标本在电子辐照下充电情况的程序。充电情况可通过照明光束的扩展、电子衍射图案的模糊以及标本上的束斑“足迹”来观察。在离焦电子衍射模式下也能看到放电现象。我们研究了多种因素对充电幅度和可见性的影响。当相邻碳膜的一部分包含在辐照标本区域时，充电现象会有所减少。
