Lütjen-Drecoll E, Lönnerholm G, Eichhorn M
Graefes Arch Clin Exp Ophthalmol. 1983;220(6):285-91. doi: 10.1007/BF00231357.
The distribution of carbonic anhydrase (CA) in the eye of man and three species of monkeys was studied by light and electron microscopy using the histochemical method of Hansson. Carbonic anhydrase staining was found in the corneal endothelium. In monkeys the endothelial cells covering the inner surface of the operculum were also stained, whereas in the human the staining stopped at Schwalbe's line. In the trabecular meshwork no cells exhibited CA staining. The iris dilator muscle showed some staining, while no clear staining was found in the pigment epithelium (PE). In the ciliary body both the PE and the non-pigmented epithelium (NPE) displayed CA staining, most prominently in the basal and lateral membranes but also discernible in the cytoplasm. The staining of the NPE (but not the PE) showed clear-cut regional differences, and the presence of CA coincided with morphological indicators of secretory activity. Heavy staining was found in the capillaries under the ciliary epithelium. In the pars plana many capillaries showed staining of only that part of the circumference of the vessel wall which faced the epithelium. Unstained segments were also found in many choroidal capillaries under the pigmented epithelium of the retina. In the retina itself CA stain was found in the pigmented epithelium, in the Müller cells and in capillaries supplying the inner retina (only monkey retina was studied).(ABSTRACT TRUNCATED AT 250 WORDS)
采用汉森组织化学方法，通过光学显微镜和电子显微镜研究了人类及三种猴子眼睛中碳酸酐酶（CA）的分布。在角膜内皮细胞中发现了碳酸酐酶染色。在猴子中，覆盖睑裂内层表面的内皮细胞也有染色，而在人类中，染色止于施瓦贝线。小梁网中没有细胞显示出CA染色。虹膜开大肌有一些染色，而色素上皮（PE）中未发现明显染色。在睫状体中，PE和无色素上皮（NPE）均显示CA染色，最明显的是在基底膜和侧膜，但在细胞质中也可辨别。NPE（而非PE）的染色显示出明显的区域差异，且CA的存在与分泌活动的形态学指标一致。在睫状体上皮下的毛细血管中发现重度染色。在睫状体平坦部，许多毛细血管仅在血管壁面向上皮的圆周部分显示染色。在视网膜色素上皮下的许多脉络膜毛细血管中也发现了未染色的节段。在视网膜本身，在色素上皮、米勒细胞以及供应视网膜内层的毛细血管中发现了CA染色（仅研究了猴子视网膜）。（摘要截短于250字）
