Ueda T, Fujimori O, Tsukise A, Yamada K
2nd Department of Anatomy, Nagoya City University Medical School, Nagoya, Japan.
Histochem Cell Biol. 1998 Apr;109(4):399-407. doi: 10.1007/s004180050241.
A variety of sialic acids contained in the rat epididymis were histochemically examined by means of lectin and pre-lectin methods by light microscopy. Epididymides from adult male Sprague-Dawley rats were fixed in Bouin's fluid and routinely embedded in paraffin wax. Hydrated sections were subjected either to the lectin methods using biotinylated Limax flavus, Sambucus nigra, Sambucus sieboldiana or Maackia amurensis lectins or to the selective periodate oxidation-phenylhydrazine-thiocarbohydrazide-silver protein-physical development technique with or without saponification. The present results revealed that principal cells in the initial segment and caput contain sialic acid linked to alpha2,6-galactose/N-acetylgalactosamine, whereas those in the corpus and cauda include the sialic acid alpha2,3-galactose sequence. Narrow and clear cells involve all the types of sialic acids examined. Basal and halo cells mainly contain sialic acid alpha2,3-galactose. 8- And/or 9-O-acetylated sialic acids were predominantly distributed in principal cells of the initial segment and proximal caput. These findings are taken to indicate that various sialic acids in the epididymis could participate in different physiological functions characteristic of the regions in this organ.
采用凝集素法和凝集素前处理法，通过光学显微镜对大鼠附睾中所含的多种唾液酸进行了组织化学检查。取成年雄性斯普拉格 - 道利大鼠的附睾，用布安氏液固定，常规石蜡包埋。水化切片分别采用生物素化的黄蛞蝓凝集素、黑接骨木凝集素、西伯利接骨木凝集素或山槐凝集素进行凝集素法检测，或采用选择性高碘酸盐氧化 - 苯肼 - 硫代碳酰肼 - 银蛋白 - 物理显影技术（有无皂化处理）。目前的结果显示，起始段和头部的主细胞含有与α2,6 - 半乳糖/ N - 乙酰半乳糖胺相连的唾液酸，而体部和尾部的主细胞则含有唾液酸α2,3 - 半乳糖序列。窄细胞和亮细胞包含所有检测类型的唾液酸。基底细胞和晕细胞主要含有唾液酸α2,3 - 半乳糖。8 - 和/或9 - O - 乙酰化唾液酸主要分布在起始段和近端头部的主细胞中。这些发现表明附睾中的各种唾液酸可能参与了该器官不同区域特有的不同生理功能。
