Lakshmanan N, Lamont S J
Department of Animal Science, Iowa State University, Ames 50011, USA.
Poult Sci. 1998 Apr;77(4):538-41. doi: 10.1093/ps/77.4.538.
Although the influence of the chicken classical MHC in resistance to many diseases is well established, the role of the recently identified, genetically independent, MHC-like region known as Rfp-Y is unclear. The objectives of this study were to analyze the frequencies of DNA polymorphisms of the Rfp-Y region in White Leghorn lines, which were divergently selected in replicate for multitrait immunocompetence, and to determine the association of these polymorphisms with Marek's disease (MD) resistance. Chicks, either with or without herpes virus of turkey (HVT) vaccination, were challenged with 500 ffu of a very virulent Marek's disease virus (Md5) at 2 d of age. The MD-related data were collected for 10 wk. PvuII-digested genomic DNA was hybridized with an Rfp-Y region-specific probe, 18.1. Three Rfp-Y polymorphisms were observed. The frequency of one Rfp-Y polymorphism was significantly different between divergently selected multitrait immunocompetence lines in one replicate only; therefore, the impact of multitrait immunocompetence selection on Rfp-Y polymorphisms is inconclusive. The PvuII defined Rfp-Y region polymorphisms had no association with either innate or vaccine-induced MD resistance to Md5 virus challenge.
虽然鸡的经典主要组织相容性复合体（MHC）在抵抗多种疾病中的作用已得到充分证实，但最近发现的、基因独立的、类似MHC的区域（称为Rfp - Y）的作用尚不清楚。本研究的目的是分析白来航鸡品系中Rfp - Y区域DNA多态性的频率，这些品系在多性状免疫能力方面进行了重复的差异选择，并确定这些多态性与马立克氏病（MD）抗性的关联。在2日龄时，对接种或未接种火鸡疱疹病毒（HVT）的雏鸡用500个蚀斑形成单位（ffu）的超强毒马立克氏病病毒（Md5）进行攻毒。收集了10周的MD相关数据。用Rfp - Y区域特异性探针18.1与经PvuII消化的基因组DNA进行杂交。观察到三种Rfp - Y多态性。仅在一个重复中，差异选择的多性状免疫能力品系之间一种Rfp - Y多态性的频率存在显著差异；因此，多性状免疫能力选择对Rfp - Y多态性的影响尚无定论。PvuII定义的Rfp - Y区域多态性与对Md5病毒攻毒的先天或疫苗诱导的MD抗性均无关联。
