Assem E S, Vickers M R
Clin Allergy. 1975 Mar;5(1):43-57. doi: 10.1111/j.1365-2222.1975.tb01835.x.
The role of three penicillin metabolites in human allergic reaction to penicillin has been assessed using sensitive in vitro tests in addition to skin testing. Conjugates of the benzylpenicilloyl (BPO), benzylpenicillenate (BPE) and penicillamine (PA) groups with human serum albumin (HSA) and bovine gamma globulin (BGG) have been made. In the passive haemagglutination test antibodies specific for all three determinants were found but there was no difference between allergic and non-allergic groups. However, the ratio IgG/IgM, specific for each determinant, was higher in normals than in allergics. In skin tests, histamine release from leucocytes and the lymphocyte transformation test, the BPO group was confirmed as the major determinant, but in some individuals the BPE or PA groups are more important.
除皮肤试验外，还通过灵敏的体外试验评估了三种青霉素代谢产物在人类青霉素过敏反应中的作用。已制备了苄青霉素酰基（BPO）、苄青霉素酸酯（BPE）和青霉胺（PA）基团与人血清白蛋白（HSA）和牛γ球蛋白（BGG）的结合物。在被动血凝试验中，发现了对所有三种决定簇均具有特异性的抗体，但过敏组和非过敏组之间没有差异。然而，针对每个决定簇的IgG/IgM比值在正常人中高于过敏者。在皮肤试验、白细胞组胺释放试验和淋巴细胞转化试验中，BPO基团被确认为主要决定簇，但在某些个体中，BPE或PA基团更为重要。
