Kimura M, Kapás L, Krueger J M
Institute for Medical and Dental Engineering, Tokyo Medical and Dental University, Japan.
Brain Res Bull. 1998 Apr;45(6):545-8. doi: 10.1016/s0361-9230(97)00441-3.
Glutatione is implicated in sleep regulation. There are circadian changes in brain glutathione levels, and nocturnal intracerebroventricular (i.c.v.) slow infusion of oxidized glutathione (GSSG) or reduced glutathione (GSH) promotes rapid-eye-movement sleep (REMS) and non-REMS (NREMS) in rats. In the present experiments, we tested the effects of GSSG on duration of sleep, NREMS intensity, and brain temperature in another species, rabbits. Male New Zealand rabbits were injected with isotonic NaCl on a baseline day and one dose of GSSG on the test day [0.15, 1.5, 15, and 150 microg/rabbit, i.c.v., or 1.5 or 15 mg/kg intravenously (i.v.)]. Electroencephalogram (EEG), motor activity, and brain temperature were recorded for 6 h. Injection of 15 microg GSSG i.c.v. significantly increased the time spent in NREMS in the first 3 h after the injection. Injection of 0.15, 1.5, and 150 microg i.s.v. GSSG, as well as systemic injections of GSSG did not affect NREMS. Intensity of NREMS as measured by EEG slow-wave activity during NREMS, and brain temperature were not affected by any of the treatments. These results are consistent with the hypothesis that glutathione may be a sleep-inducing factor in the brain.
谷胱甘肽与睡眠调节有关。大脑谷胱甘肽水平存在昼夜变化，夜间脑室内（i.c.v.）缓慢输注氧化型谷胱甘肽（GSSG）或还原型谷胱甘肽（GSH）可促进大鼠快速眼动睡眠（REMS）和非快速眼动睡眠（NREMS）。在本实验中，我们测试了GSSG对另一种动物兔子的睡眠时间、NREMS强度和脑温的影响。雄性新西兰兔在基线日注射等渗氯化钠，在测试日注射一剂GSSG[0.15、1.5、15和150微克/兔，脑室内注射，或1.5或15毫克/千克静脉注射（i.v.）]。记录脑电图（EEG）、运动活动和脑温6小时。脑室内注射15微克GSSG显著增加了注射后前3小时NREMS的时间。脑室内注射0.15、1.5和150微克GSSG以及全身注射GSSG均未影响NREMS。NREMS期间通过EEG慢波活动测量的NREMS强度和脑温不受任何处理的影响。这些结果与谷胱甘肽可能是大脑中一种诱导睡眠的因子这一假设一致。
