Junquera C, Martínez-Ciriano C, Castiella T, Aisa J, Blasco J, Peg M T, Azanza M J
Dpto. de Ciencias Morfológicas, Facultad de Medicina de Zaragoza, Spain.
Neurochem Res. 1998 Apr;23(4):493-504. doi: 10.1023/a:1022474316475.
We study the esophagus of Podarcis hispanica through different methods to clarify the structure and affinities of its wall innervation. The acetylcholinesterase method reveals cholinesterase activity in two submucosal nervous plexuses, with an increasing degree of structural complexity in the reptilian esophagus, compared with amphibians. Noradrenergic innervation, detected through fluorescence induced by formol, widely spreads its network in both the myenteric and submucosal plexuses (around the blood vessels in the external submucosal plexus, and to the glandular lamina propria in the inner submucosal plexus). Immunohistochemistry for vasoactive intestinal peptide shows a widespread innervation, with neurons clustered in ganglia and also scattered through the VIPergic network, only at the myenteric plexus. Immunohistochemistry for substance P shows a rich innervation along the entire wall of the esophagus, more concentrated in its caudal region, around the blood vessels. Electron microscopy shows the enteric neuronal ultrastructure and its relationship with the esophagus wall.
我们通过不同方法研究了西班牙壁蜥的食管，以阐明其壁内神经支配的结构和亲缘关系。乙酰胆碱酯酶法显示在两个黏膜下神经丛中有胆碱酯酶活性，与两栖动物相比，爬行动物食管的结构复杂性程度增加。通过福尔马林诱导的荧光检测到的去甲肾上腺素能神经支配，其网络广泛分布于肌间神经丛和黏膜下神经丛（在外黏膜下神经丛的血管周围，以及在内黏膜下神经丛的腺固有层）。血管活性肠肽的免疫组织化学显示神经支配广泛，神经元聚集在神经节中，也散布在仅在肌间神经丛的血管活性肠肽能网络中。P物质的免疫组织化学显示食管整个壁内神经支配丰富，在其尾部区域、血管周围更为集中。电子显微镜显示了肠神经元的超微结构及其与食管壁的关系。
