Holmgren S, Nilsson S
Cell Tissue Res. 1983;234(3):595-618. doi: 10.1007/BF00218654.
The presence of peptides and 5-hydroxytryptamine (5-HT) in neurons and endocrine cells in the gastrointestinal tract of the spiny dogfish, Squalus acanthias, was investigated by means of immunohistochemistry, and the distribution of catecholamines by use of the Falck-Hillarp fluorescence-histochemical technique. Bombesin-like immunoreactivity was present in numerous nerves in all layers and all parts of the gut, and also in endocrine cells in the mucosa throughout the stomach, rectum and intestine. VIP-like immunoreactivity occurred in an abundance of nerve fibres and in nerve cell bodies in all parts of the gut except the oesophagus, while 5-HT-like immunoreactivity was found sparsely in nerve fibres and more frequently in endocrine cells throughout the gut. Gastrin/CCK-like immunoreactivity was present in numerous nerve fibres in the rectum, but only in scattered fibres in the other parts of the gut. Endocrine cells showing gastrin/CCK-like immunoreactivity were present in the intestine only. Somatostatin-like immunoreactivity occurred in both nerve fibres and endocrine cells of the stomach and intestine, but only in nerves in the rectum. Neurotensin-like immunoreactivity was confined to endocrine cells of the intestine. Falck-Hillarp fluorescence histochemistry revealed 5-HT in endocrine cells and catecholamines in nerve fibres (and possibly also in endocrine cells) throughout the gut. Bombesin-, VIP-, gastrin/CCK- and somatostatin-like immunoreactivities and catecholamine fluorescence were present in nerve fibres of the rectal gland and, with the exception of gastrin/CCK-like immunoreactivity, also in nerve bundles in the walls of the coeliac and mesenteric arteries. The findings of the present study form an anatomical basis for the assumption that several of the neuropeptides and amines could function as neurotransmitters or neuromodulators in the gut of Squalus.
运用免疫组织化学方法研究了棘鲨（Squalus acanthias）胃肠道神经元和内分泌细胞中肽类和5-羟色胺（5-HT）的存在情况，并使用福尔克-希拉尔普荧光组织化学技术研究了儿茶酚胺的分布。蛙皮素样免疫反应性存在于肠道各层和各部位的众多神经中，也存在于整个胃、直肠和肠道黏膜的内分泌细胞中。血管活性肠肽样免疫反应性存在于除食管外肠道各部位的大量神经纤维和神经细胞体中，而5-HT样免疫反应性在神经纤维中分布稀疏，在整个肠道的内分泌细胞中更为常见。胃泌素/胆囊收缩素样免疫反应性存在于直肠的众多神经纤维中，但在肠道其他部位仅存在于散在的纤维中。显示胃泌素/胆囊收缩素样免疫反应性的内分泌细胞仅存在于肠道中。生长抑素样免疫反应性存在于胃和肠道的神经纤维和内分泌细胞中，但仅存在于直肠的神经中。神经降压素样免疫反应性仅限于肠道的内分泌细胞。福尔克-希拉尔普荧光组织化学显示，整个肠道的内分泌细胞中有5-HT，神经纤维（可能也在内分泌细胞中）中有儿茶酚胺。蛙皮素、血管活性肠肽、胃泌素/胆囊收缩素和生长抑素样免疫反应性以及儿茶酚胺荧光存在于直肠腺的神经纤维中，除胃泌素/胆囊收缩素样免疫反应性外，也存在于腹腔动脉和肠系膜动脉壁的神经束中。本研究结果为几种神经肽和胺类可能在棘鲨肠道中作为神经递质或神经调节剂发挥作用这一假设提供了解剖学基础。
