Søndergaard K, Stengaard-Pedersen K, Zachariae H, Heickendorff L, Deleuran M, Deleuran B
Department of Rheumatology, Aarhus University Hospital, Denmark.
Br J Rheumatol. 1998 Mar;37(3):304-10. doi: 10.1093/rheumatology/37.3.304.
In order to investigate whether soluble intercellular adhesion molecule-1 (sICAM-1) and soluble interleukin-2 receptors (sIL-2R) were present in scleroderma skin, and to compare their levels to concentrations measured in plasma and clinical parameters, we examined suction blister fluid and plasma from 13 patients with systemic sclerosis and 11 healthy volunteers. Suction blisters and biopsies were from the transition zone between normal skin and scleroderma, and uninvolved abdominal skin. The levels of sICAM-1 and sIL-2R were significantly increased in both plasma and suction blister fluid from systemic sclerosis patients compared with healthy volunteers. ICAM-1 was localized to vessels and perivascular mononuclear infiltrates by immunohistochemical methods. IL-2R was expressed by CD3-positive cells. The elevated levels of sICAM-1 and sIL-2R in suction blister fluid point towards activation of endothelial cells and T cells in both the transition zone and uninvolved skin of systemic sclerosis patients.
为了研究可溶性细胞间黏附分子-1（sICAM-1）和可溶性白细胞介素-2受体（sIL-2R）是否存在于硬皮病皮肤中，并将它们的水平与血浆中测得的浓度及临床参数进行比较，我们检测了13例系统性硬化症患者和11名健康志愿者的水疱抽吸液和血浆。水疱抽吸和活检取材于正常皮肤与硬皮病之间的过渡区以及未受累的腹部皮肤。与健康志愿者相比，系统性硬化症患者血浆和水疱抽吸液中sICAM-1和sIL-2R的水平均显著升高。通过免疫组织化学方法将ICAM-1定位于血管和血管周围单核浸润处。IL-2R由CD3阳性细胞表达。系统性硬化症患者过渡区和未受累皮肤中水疱抽吸液中sICAM-1和sIL-2R水平的升高表明内皮细胞和T细胞被激活。
