小鼠-人异核体细胞表面抗原的扩散速率。I. 群体分析。
Diffusion rates of cell surface antigens of mouse-human heterokaryons. I. Analysis of the population.
作者信息
Edidin M, Wei T Y
出版信息
J Cell Biol. 1977 Nov;75(2 Pt 1):475-82. doi: 10.1083/jcb.75.2.475.
Abstract
The rate of appearance, in a newly formed heterokaryon population, of cells bearing completely intermixed mouse and human surface antigens may be used to estimate diffusion constants for antigens on individual cells. From this estimate, it appears that the surface antigens in most cells do not diffuse at the rate expected, but rather move more slowly, by a factor of ten or more, than expected from either measured or calculated diffusion constants for proteins freely mobile in the plane of a lipid membrane. Differences in diffusion rates between cells are not due to effects of Sendai virus, or of trypsin. Restrictions on diffusion are apparently not due to cytochalasin B- or Colcemid-sensitive elements.
摘要
在新形成的异核体群体中，带有完全混合的小鼠和人类表面抗原的细胞出现的速率，可用于估计单个细胞上抗原的扩散常数。根据这一估计，似乎大多数细胞中的表面抗原扩散速率并不像预期的那样，而是比在脂质膜平面上自由移动的蛋白质的测量或计算扩散常数所预期的要慢十倍或更多倍。细胞间扩散速率的差异不是由仙台病毒或胰蛋白酶的作用引起的。对扩散的限制显然不是由细胞松弛素B或秋水仙酰胺敏感元件引起的。
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