Ember I, Kiss I, Pusztai Z
Department of Preventive Medicine, University Medical School of Pecs, Hungary.
Anticancer Res. 1998 Jan-Feb;18(1A):445-7.
Dimethylbenz[a]anthracene is a pluripotent carcinogenic chemical, which acts as an initiator by causing point mutations in certain oncogenes and tumor suppressor genes. Changes in their expression may be another possible area of investigation the carcinogenic effect of DMBA. Elevated expression of oncogenes was previously has been shown after treatment with carcinogenic compounds. In the present study, expression of c-myc, c-Ha-ras and p53 24 hours after a single dose treatment of DMBA in the spleen and in the liver of Long-Evans rats was investigated. Control animals were injected with the solvent corn oil only. We could not find any significant change on the transcriptional level of the investigated oncogenes in the liver. In the spleen, the overexpression of Ha-ras was 2-fold and c-myc was 3-fold higher in the DMBA-treated rats than in the corresponding control group. Since DMBA is a typical environmental carcinogen, the results of animal experiments may serve as a basis for application of gene expression investigations as a screening method in humans.
二甲基苯并[a]蒽是一种多能致癌化学物质，它通过在某些癌基因和肿瘤抑制基因中引起点突变而作为引发剂。它们表达的变化可能是研究二甲基苯并[a]蒽致癌作用的另一个可能领域。先前已显示用致癌化合物处理后癌基因表达升高。在本研究中，研究了在单次剂量给予二甲基苯并[a]蒽后24小时，Long-Evans大鼠脾脏和肝脏中c-myc、c-Ha-ras和p53的表达。对照动物仅注射溶剂玉米油。我们在肝脏中未发现所研究癌基因转录水平有任何显著变化。在脾脏中，经二甲基苯并[a]蒽处理的大鼠中Ha-ras的过表达比相应对照组高2倍，c-myc高3倍。由于二甲基苯并[a]蒽是一种典型的环境致癌物，动物实验结果可作为将基因表达研究作为人类筛查方法应用的基础。
