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Chromatographic analysis of polysaccharides extracted from Chinese Indocalamus tesselatus.

作者信息

Ding Y Q, Li Y, Chen C Y, Elmahadi E A, Xu H B

机构信息

Experimental Centre of Nanyang Institute of Science and Technology, China.

出版信息

Biomed Chromatogr. 1998 Mar-Apr;12(2):86-8. doi: 10.1002/(SICI)1099-0801(199803/04)12:2<86::AID-BMC728>3.0.CO;2-T.

DOI:10.1002/(SICI)1099-0801(199803/04)12:2<86::AID-BMC728>3.0.CO;2-T
PMID:9568276
Abstract

Three fractions of polysaccharides that have high biological activity were extracted from Chinese bamboo leaves (Indocalamus Tesselatus) with 0.9% sodium chloride, 1% ammonium oxalate and 85% ethanol. Their component sugars were investigated by paper chromatography developed with n-butanol-pyridine-water (6:4:3 by vol), and by gas chromatography with crown ester stationary-phase-fused silica capillary column. Their monosaccharide compositions are different from each other with glucuronic acid, galactose, arabinose, mannose, glucose, and xylose (52, 18, 9, 7 and 6%, respectively) in one fraction, only glucuronic acid in another and glucuronic acid, fucose, arabinose and xylose (36, 31, 24 and 9%, respectively) in the third fraction. The protection effect of two polysaccharides against experimental hepatitis was studied in mice.

摘要

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