Giunter E A, Popeĭko O V, Ovodov Iu S
Institute of Physiology, Komi Research Center, Ural Division of the Russian Academy of Sciences, Syktyvkar, 167982 Russia.
Prikl Biokhim Mikrobiol. 2004 Jan-Feb;40(1):94-7.
Two fractions that included acid arabinogalactan and pectin were extracted from the callus culture of duckweed plants (Lemna minor L.) with water and ammonium oxalate. Residues of galactose and arabinose in the 2.0-2.5:1 ratio were the major constituents of acid arabinogalactan. The pectin fraction contained primarily residues of glucuronic acids, galactose, and arabinose. The percentage of arabinogalactan and pectin was similar. The yield of polysaccharide fractions did not depend on the method for their isolation. Extraction with water, treatment of the biomass with an aqueous solution of formalin and diluted hydrochloric acid, and extraction with an aqueous solution of ammonium oxalate allowed us to obtain the highest-purity pectin polysaccharide.
用水和草酸铵从浮萍（Lemna minor L.）愈伤组织培养物中提取了两种成分，其中包括酸性阿拉伯半乳聚糖和果胶。半乳糖和阿拉伯糖的残留比例为2.0 - 2.5:1，是酸性阿拉伯半乳聚糖的主要成分。果胶部分主要含有葡萄糖醛酸、半乳糖和阿拉伯糖的残留物。阿拉伯半乳聚糖和果胶的百分比相似。多糖部分的产量不取决于其分离方法。用水提取、用福尔马林水溶液和稀盐酸处理生物质以及用草酸铵水溶液提取，使我们能够获得纯度最高的果胶多糖。
