虹鳟（Salmo gairdneri R.）鳃中的AMP脱氨酶。细胞蛋白水解酶的激活方式。

AMP deaminase in the gill of trout (Salmo gairdneri R.). Modalities of an activation by cellular proteolytic enzymes.

Raffin J P, Leray C

Laboratoire de Physiologie comparée des Regulations, C.N.R.S., - B.P.CR, 67037 Strasbourg-Cédex, France.

Comp Biochem Physiol B. 1979;62(1):23-9. doi: 10.1016/0305-0491(79)90006-3.

Abstract

The AMP deaminase activity present in a crude extract of trout gill increased with time. 2. Soybean trypsin inhibitor and alpha2-macroglobulin inhibited the AMP deaminase activation. NEM was also inhibitory at 10-3 M. 3. The activation process is followed by a decrease of activity which is inhibited by EGTA and enhanced by Mg2+. These two compounds were without effect on the activation process itself. 4. Trypsin induces a sharp activation of AMP deaminase in a fresh gill extract but is without effect on a fully activated extract. 5. Overall, the results suggest that neutral proteinases are implicated in AMP deaminase activation.

摘要

虹鳟鱼鳃粗提物中的AMP脱氨酶活性随时间增加。2. 大豆胰蛋白酶抑制剂和α2-巨球蛋白抑制AMP脱氨酶的激活。NEM在10-3 M时也具有抑制作用。3. 激活过程之后是活性降低，EGTA可抑制该活性降低，而Mg2+可增强该活性降低。这两种化合物对激活过程本身没有影响。4. 胰蛋白酶可在新鲜鳃提取物中诱导AMP脱氨酶的急剧激活，但对完全激活的提取物没有影响。5. 总体而言，结果表明中性蛋白酶与AMP脱氨酶的激活有关。