Richardson M D, Brownlie C E, Shankland G S
Department of Dermatology, University of Glasgow, UK.
J Med Vet Mycol. 1992;30(6):433-41.
Little is known about effector cell modulation regarding phagocytosis and candidacidal activities of human neutrophils in response to granulocyte-macrophage colony stimulating factor (GM-CSF). In neutrophil monolayer assays, pre-treatment of cells with ultrapure GM-CSF at 40 Units ml-1 for a minimum of 1 h showed a significant enhancement of phagocytosis of Candida albicans (phagocytic index of 2.6 compared with 1.6 for unactivated neutrophils) after a 20 min ingestion period (P < 0.001). By counting viable colonies 50% of intracellular yeast cells were killed by non-activated neutrophils following internalization for 2 h. Neutrophils activated for 2 h with 40 Units ml-1 of GM-CSF killed 90% of intracellular blastoconidia (P < 0.02). These results demonstrate that in in vitro assays GM-CSF enhances phagocytosis and intracellular killing of C. albicans.
关于粒细胞-巨噬细胞集落刺激因子（GM-CSF）对人中性粒细胞吞噬作用和杀念珠菌活性的效应细胞调节作用，人们了解甚少。在中性粒细胞单层试验中，用40单位/毫升的超纯GM-CSF预处理细胞至少1小时，在20分钟的摄取期后，白色念珠菌的吞噬作用显著增强（吞噬指数为2.6，未激活的中性粒细胞为1.6）（P<0.001）。通过计数活菌落，50%的内化2小时后的细胞内酵母细胞被未激活的中性粒细胞杀死。用40单位/毫升的GM-CSF激活2小时的中性粒细胞杀死了90%的细胞内芽生孢子（P<0.02）。这些结果表明，在体外试验中，GM-CSF增强了白色念珠菌的吞噬作用和细胞内杀伤作用。
