Cavaliere V, Taddei C, Gargiulo G
Laboratorio di Genetica Molecolare - Dipartimento di Biologia Evoluzionistica Sperimentale, Università di Bologna, Via Selmi 3, I-40126 Bologna, Italy.
Dev Genes Evol. 1998 Apr;208(2):106-12. doi: 10.1007/s004270050160.
In Drosophila a remarkable feature of oogenesis is the regression of the nurse cells after dumping their cytoplasmic contents into the oocyte. We have studied the nature of this process at the late stages of egg chamber development. In egg chambers DAPI staining shows highly condensed chromatin from stage 12 and TUNEL labelling shows DNA fragmentation up to stage 14. Gel electrophoresis of the end-labelled DNA, extracted from isolated egg chambers at the same stages of development, shows a ladder typical of apoptotic nuclei. This provides evidence that, during Drosophila oogenesis, the nurse cells undergo apoptosis. Apoptotic nuclei have also been detected in dumping-defective egg chambers, indicating that the cytoplasmic depletion of nurse cells is concurrent with but apparently not the cause of the process.
在果蝇中，卵子发生的一个显著特征是滋养细胞在将其细胞质内容物倾倒入卵母细胞后发生退化。我们研究了卵室发育后期这一过程的本质。在卵室中，DAPI染色显示从12期开始染色质高度浓缩，TUNEL标记显示直到14期都有DNA片段化。对从处于相同发育阶段的分离卵室中提取的末端标记DNA进行凝胶电泳，显示出典型的凋亡细胞核梯状条带。这提供了证据，表明在果蝇卵子发生过程中，滋养细胞会经历凋亡。在倾倒缺陷的卵室中也检测到了凋亡细胞核，这表明滋养细胞的细胞质消耗与该过程同时发生，但显然不是该过程的原因。
