School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, PR China.
Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology, South China Normal University, Guangzhou, PR China.
Cell Death Dis. 2022 Sep 2;13(9):756. doi: 10.1038/s41419-022-05195-9.
In Drosophila ovary, niche is composed of somatic cells, including terminal filament cells (TFCs), cap cells (CCs) and escort cells (ECs), which provide extrinsic signals to maintain stem cell renewal or initiate cell differentiation. Niche establishment begins in larval stages when terminal filaments (TFs) are formed, but the underlying mechanism for the development of TFs remains largely unknown. Here we report that transcription factor longitudinals lacking (Lola) is essential for ovary morphogenesis. We showed that Lola protein was expressed abundantly in TFCs and CCs, although also in other cells, and lola was required for the establishment of niche during larval stage. Importantly, we found that knockdown expression of lola induced apoptosis in adult ovary, and that lola affected adult ovary morphogenesis by suppressing expression of Regulator of cullins 1b (Roc1b), an apoptosis-related gene that regulates caspase activation during spermatogenesis. These findings significantly expand our understanding of the mechanisms controlling niche establishment and adult oogenesis in Drosophila.
在果蝇卵巢中，龛位由体细胞组成，包括末端丝细胞（TFCs）、帽细胞（CCs）和护送细胞（ECs），它们提供外在信号以维持干细胞更新或启动细胞分化。龛位的建立始于幼虫期，此时形成末端丝（TFs），但 TFs 发育的潜在机制在很大程度上仍是未知的。在这里，我们报告转录因子纵向缺失（Lola）对于卵巢形态发生是必需的。我们表明，Lola 蛋白在 TFCs 和 CCs 中大量表达，尽管在其他细胞中也有表达，并且在幼虫期龛位的建立中需要 lola。重要的是，我们发现 lola 的敲低表达诱导了成虫卵巢中的细胞凋亡，并且 lola 通过抑制凋亡相关基因 Regulator of cullins 1b（Roc1b）的表达来影响成虫卵巢形态发生，该基因在精子发生过程中调节半胱天冬酶的激活。这些发现显著扩展了我们对控制果蝇龛位建立和成虫卵子发生的机制的理解。
