Mills G, Earnshaw R, Patterson M F
Department of Food Science (Food Microbiology), Queen's University of Belfast, UK.
Lett Appl Microbiol. 1998 Mar;26(3):227-30. doi: 10.1046/j.1472-765x.1998.00329.x.
Spores of Clostridium sporogenes were found to be resistant to ultra high pressure, with treatments of 600 MPa for 30 min at 20 degrees C causing no significant inactivation. Combination treatments including heat and pressure applied simultaneously (e.g. 400 MPa at 60 degrees C for 30 min) or sequentially (e.g. 80 degrees C for 10 min followed by 400 MPa for 30 min) proved more effective at inactivating spores. Pressure cycling (e.g. 60 MPa followed by 400 MPa at 60 degrees C) also reduced spore numbers. Overall, these pressure treatments resulted in less than a 3 log reduction, and it was concluded that the spores could not be inactivated by pressure alone. This could indicate that for the effective inactivation of bacterial spores, high pressure technology may have to be used in combination with other preservation methods.
研究发现，生孢梭菌的孢子对超高压具有抗性，在20℃下用600兆帕处理30分钟不会导致显著失活。同时施加加热和压力（例如在60℃下400兆帕处理30分钟）或依次施加（例如80℃处理10分钟，随后400兆帕处理30分钟）的联合处理在使孢子失活方面更有效。压力循环（例如60兆帕，随后在60℃下400兆帕）也能减少孢子数量。总体而言，这些压力处理导致孢子数量减少不到3个对数级，得出的结论是仅靠压力无法使孢子失活。这可能表明，为了有效灭活细菌孢子，高压技术可能必须与其他保存方法结合使用。
