Cohen J, Scott R, Alikani M, Schimmel T, Munné S, Levron J, Wu L, Brenner C, Warner C, Willadsen S
The Institute for Reproductive Medicine and Science of Saint Barnabas, Livingston, New Jersey, USA.
Mol Hum Reprod. 1998 Mar;4(3):269-80. doi: 10.1093/molehr/4.3.269.
Ooplasmic transplantation aimed at restoring normal growth in developmentally compromised oocytes and embryos was evaluated in seven couples (eight cycles) with multiple implantation failures. Two approaches were investigated to transfer ooplasm from donor eggs at metaphase II (MII) stage into patient MII eggs: (i) electrofusion of a ooplasmic donor fragment into each patient egg (three cycles), and (ii) direct injection of a small amount of ooplasm from a donor egg into each patient egg (five cycles). Some donor eggs were used multiple times. Donor eggs were divided into two groups, one being used for ooplasmic extraction and the other one for egg donation. Cleaved embryos resulting from the latter were cryopreserved, where numbers and satisfactory development permitted. A second control group consisted of embryos derived from patient eggs after intracytoplasmic sperm injection without ooplasmic transfer. This was performed when sufficient number of eggs were available (n = 5). Donor eggs (n = 40) were evaluated cytogenetically after micromanipulation in order to confirm the presence of chromosomes. One egg was anuclear and the recipient embryos were not transferred. Normal fertilization was significantly higher after injection of ooplasm (63%) in comparison with fusion (23%). Pronuclear anomalies appeared enhanced after fusion with ooplasts. Embryo morphology was not improved in the three cycles with electrofusion and patients did not become pregnant. An improvement in embryo morphology was noted in two patients after injection of ooplasm and both became pregnant, but one miscarried. A third pregnancy was established in the repeat patient, without obvious embryo improvement. One baby was born and the third pregnancy is ongoing with a normal karyotype. Two other patients with male factor infertility had poor embryos after ooplasmic injection, but the donor embryo controls were also poor. The patients did not become pregnant and had no donor embryos frozen. Ooplasmic transfer at the MII stage may be promising in patients with compromised embryos; however, evaluation of ooplasmic anomalies and optimization of techniques will require further investigation prior to widescale application.
对7对夫妇（8个周期）多次植入失败的情况进行了旨在恢复发育受损卵母细胞和胚胎正常生长的卵质移植评估。研究了两种将处于减数分裂中期II（MII）期的供体卵母细胞的卵质转移到患者MII期卵母细胞中的方法：（i）将卵质供体片段电融合到每个患者卵母细胞中（3个周期），以及（ii）将少量供体卵母细胞的卵质直接注射到每个患者卵母细胞中（5个周期）。一些供体卵母细胞被多次使用。供体卵母细胞分为两组，一组用于卵质提取，另一组用于卵子捐赠。由后者产生的分裂胚胎在数量和发育情况允许时进行冷冻保存。第二个对照组由在未进行卵质转移的情况下通过胞浆内单精子注射从患者卵母细胞获得的胚胎组成。当有足够数量的卵母细胞时进行此项操作（n = 5）。为了确认染色体的存在，对40个供体卵母细胞在显微操作后进行了细胞遗传学评估。有一个卵母细胞无细胞核，未移植受体胚胎。与融合（23%）相比，注射卵质后的正常受精率显著更高（63%）。与卵质体融合后原核异常似乎增加。在电融合的3个周期中胚胎形态没有改善，患者也未怀孕。在注射卵质后，有两名患者的胚胎形态得到改善，两人都怀孕了，但其中一人流产。在重复治疗的患者中建立了第三次妊娠，胚胎无明显改善。一名婴儿出生，第三次妊娠正在进行，核型正常。另外两名男性因素不育患者在注射卵质后胚胎质量差，但供体胚胎对照也很差。患者未怀孕，也没有冷冻供体胚胎。MII期卵质转移对胚胎受损的患者可能有前景；然而，在广泛应用之前，需要进一步研究卵质异常的评估和技术的优化。
