一种章鱼毒素，即黄斑毒素，能选择性地阻断鱿鱼轴突中的钠电流。
An octopus toxin, maculotoxin, selectively blocks sodium current in squid axons.
作者信息
Gage P W, Moore J W, Westerfield M
出版信息
J Physiol. 1976 Jul;259(2):427-43. doi: 10.1113/jphysiol.1976.sp011474.
Abstract
- A low molecular weight, stable, cationic neurotoxin (maculotoxin, MTX) extracted from the posterior salivary glands of the octopus Hapalochlaena maculosa, blocked sodium current in voltage-clamped squid axons without affecting potassium current. 2. The effectiveness of MTX was increased by repetitive, brief, depolarizing pulses but not by a single prolonged depolarization. 3. The potency of MTX decreased at pHs from 8 to 9. Effectiveness could be restored be restored by lowering the pH to 7-1 again. It was concluded that MTX is active in its cationic form. 4. MTX affected sodium conductance kinetics, slowing the turn-on of sodium current. This effect was most noticeable with small deploarizations but became progressively less with larger depolarizations. Neither the turn-off of sodium current nor sodium inactivation kinetics were affected by the toxin. 5. MTX inhibited sodium current without inhibiting sodium gating current. 6. The effectiveness of MTX was not detectably changed when calcium concentration was varied from 50 to 10 mM, or sodium concentration was varied from 225 to 750 mM.
摘要
- 从黄斑海蛞蝓（Hapalochlaena maculosa）后唾液腺中提取的一种低分子量、稳定的阳离子神经毒素（黄斑毒素，MTX），可阻断电压钳制的鱿鱼轴突中的钠电流，而不影响钾电流。2. 重复性的短暂去极化脉冲可增强MTX的作用效果，但单次长时间去极化则不能。3. MTX的效力在pH值从8降至9时降低。将pH值再次降至7.1可恢复其效力。由此得出结论，MTX以阳离子形式具有活性。4. MTX影响钠电导动力学，减缓钠电流的开启。这种效应在小幅度去极化时最为明显，但随着去极化幅度增大而逐渐减弱。毒素对钠电流的关闭或钠失活动力学均无影响。5. MTX抑制钠电流，但不抑制钠门控电流。6. 当钙浓度从50 mM变化至10 mM，或钠浓度从225 mM变化至750 mM时，MTX的效力未出现可检测到的变化。
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