Suppr超能文献

人类骨髓CD34(+)细胞上CD86的表达可识别巨噬细胞和树突状细胞具有免疫活性的定向祖细胞。

Expression of CD86 on human marrow CD34(+) cells identifies immunocompetent committed precursors of macrophages and dendritic cells.

作者信息

Ryncarz R E, Anasetti C

机构信息

Division of Clinical Research, Fred Hutchinson Cancer Research Center, Division of Oncology, University of Washington, Seattle, WA, USA.

出版信息

Blood. 1998 May 15;91(10):3892-900.

PMID:9573027
Abstract

Macrophages and dendritic cells derive from a hematopoietic stem cell and the existence of a common committed progenitor has been hypothesized. We have recently found in normal human marrow a subset of CD34(+) cells that constitutively expresses HLA-DR and low levels of CD86, a natural ligand for the T cell costimulation receptor CD28. This CD34(+) subset can elicit responses from allogeneic T cells. In this study, we show that CD34(+)/CD86(+) cells can also present tetanus toxoid antigen to memory CD4(+) T cells. CD86 is expressed at low levels in macrophages and high levels in dendritic cells. Therefore, we have tested the hypothesis that CD34(+)/CD86(+) cells are the common precursors of both macrophages and dendritic cells. CD34(+)/CD86(+) marrow cells cultured in granulocyte-macrophage colony-stimulating factor (GM-CSF)-generated macrophages. In contrast, CD34(+)/CD86(-) cells cultured in GM-CSF generated a predominant population of granulocytes. CD34(+)/CD86(+) cells cultured in GM-CSF plus tumor necrosis factor-alpha (TNF-alpha) generated almost exclusively CD1a+/CD83(+) dendritic cells. In contrast, CD34(+)/CD86(-) cells cultured in GM-CSF plus TNF-alpha generated a variety of cell types, including a small population of dendritic cells. In addition, CD34(+)/CD86(+) cells cultured in granulocyte colony-stimulating factor failed to generate CD15(+) granulocytes. Therefore, CD34(+)/CD86(+) cells are committed precursors of both macrophages and dendritic cells. The ontogeny of dendritic cells was recapitulated by stimulation of CD34(+)/CD86(-) cells with TNF-alpha that induced expression of CD86. Subsequent costimulation of CD86(+) cells with GM-CSF plus TNF-alpha lead to expression of CD83 and produced terminal dendritic cell differentiation. Thus, expression of CD86 on hematopoietic progenitor cells is regulated by TNF-alpha and denotes differentiation towards the macrophage or dendritic cell lineages.

摘要

巨噬细胞和树突状细胞源自造血干细胞,并且人们推测存在一种共同的定向祖细胞。我们最近在正常人骨髓中发现了一部分CD34(+)细胞,它们组成性地表达HLA-DR以及低水平的CD86,CD86是T细胞共刺激受体CD28的天然配体。这个CD34(+)亚群能够引发同种异体T细胞的反应。在本研究中,我们表明CD34(+)/CD86(+)细胞也能够将破伤风类毒素抗原呈递给记忆性CD4(+)T细胞。CD86在巨噬细胞中低水平表达,而在树突状细胞中高水平表达。因此,我们检验了CD34(+)/CD86(+)细胞是巨噬细胞和树突状细胞共同前体的假说。在粒细胞-巨噬细胞集落刺激因子(GM-CSF)中培养的CD34(+)/CD86(+)骨髓细胞生成了巨噬细胞。相反,在GM-CSF中培养的CD34(+)/CD86(-)细胞生成了以粒细胞为主的细胞群。在GM-CSF加肿瘤坏死因子-α(TNF-α)中培养的CD34(+)/CD86(+)细胞几乎只生成了CD1a+/CD83(+)树突状细胞。相反,在GM-CSF加TNF-α中培养的CD34(+)/CD86(-)细胞生成了多种细胞类型,包括少量的树突状细胞。此外,在粒细胞集落刺激因子中培养的CD34(+)/CD86(+)细胞未能生成CD15(+)粒细胞。因此,CD34(+)/CD8(+)细胞是巨噬细胞和树突状细胞的定向前体。通过用TNF-α刺激CD34(+)/CD86(-)细胞诱导CD86的表达,概括了树突状细胞的个体发生过程。随后用GM-CSF加TNF-α对CD86(+)细胞进行共刺激导致CD83的表达并产生终末树突状细胞分化。因此,造血祖细胞上CD86的表达受TNF-α调节,并表明向巨噬细胞或树突状细胞谱系的分化。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验