对大肠杆菌乳糖/H⁺共转运蛋白中第177位和第236位进行抑制子扫描，以及酸性取代基的典型效应，这表明跨膜片段相对于脂质双层存在一种有利的取向。
Suppressor scanning at positions 177 and 236 in the Escherichia coli lactose/H+ cotransporter and stereotypical effects of acidic substituents that suggest a favored orientation of transmembrane segments relative to the lipid bilayer.
作者信息
King S C, Li S
机构信息
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, Texas 77555-0641, USA.
出版信息
J Bacteriol. 1998 May;180(10):2756-8. doi: 10.1128/JB.180.10.2756-2758.1998.
Abstract
Acidic substituents for Ala-177 (helix 6) or Tyr-236 (helix 7) in LacY cause effects on sugar recognition and cosubstrate coupling that are stereotypical of neutral substituents. Thus, helices 6 and 7 are probably oriented to produce little side-chain contact with the low dielectric lipid bilayer at positions 177 and 236.
摘要
乳糖通透酶（LacY）中丙氨酸-177（螺旋6）或酪氨酸-236（螺旋7）的酸性取代基对糖识别和共底物偶联产生的影响与中性取代基的典型影响相同。因此，螺旋6和螺旋7的取向可能使得在177和236位与低介电常数的脂质双层几乎没有侧链接触。
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本文引用的文献
1
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J Biol Chem. 1997 Oct 17;272(42):26110-6. doi: 10.1074/jbc.272.42.26110.
2
Interpreting the effects of site-directed mutagenesis on active transport systems.
Biochim Biophys Acta. 1994 Jul 13;1193(1):165-78. doi: 10.1016/0005-2736(94)90346-8.
3
Structural features of the uniporter/symporter/antiporter superfamily.单向转运体/同向转运体/反向转运体超家族的结构特征。
Protein Sci. 1995 Mar;4(3):534-7. doi: 10.1002/pro.5560040319.
4
Characterization of lactose carrier mutants which transport maltose.转运麦芽糖的乳糖载体突变体的特性分析
J Biol Chem. 1985 Dec 25;260(30):16181-6.
5
lac permease of Escherichia coli: histidine-205 and histidine-322 play different roles in lactose/H+ symport.
Biochemistry. 1986 Aug 12;25(16):4483-5. doi: 10.1021/bi00364a003.
6
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Biochemistry. 1986 Aug 12;25(16):4486-8. doi: 10.1021/bi00364a004.
7
Characterization of site-directed mutants in the lac permease of Escherichia coli. 1. Replacement of histidine residues.大肠杆菌乳糖通透酶中定点突变体的特性研究。1. 组氨酸残基的替换
Biochemistry. 1989 Mar 21;28(6):2525-33. doi: 10.1021/bi00432a027.
8
9
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Annu Rev Biophys Biophys Chem. 1986;15:163-93. doi: 10.1146/annurev.bb.15.060186.001115.
10
Towards an understanding of the structural basis of 'forbidden' transport pathways in the Escherichia coli lactose carrier: mutations probing the energy barriers to uncoupled transport.
Mol Microbiol. 1990 Sep;4(9):1433-8. doi: 10.1111/j.1365-2958.1990.tb02053.x.
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