Cirera S, Aguadé M
Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Spain.
Gene. 1998 Apr 14;210(2):247-54. doi: 10.1016/s0378-1119(98)00069-9.
A 3.1-kb region of Drosphila subobscura homologous to the Acp70A region of D. melanogaster, which contains the sex-peptide gene, was cloned and sequenced. This region contains an approximately 600-bp duplication that includes the sex-peptide and its 5' and 3' flanking regions. The preproteins are 54 and 56 amino acids long, respectively (as compared to 55 amino acids in D. melanogaster), and each includes a 19-amino-acid-long signal peptide. The C-terminal part of the mature peptide is highly conserved between D. melanogaster and the two copies of D. subobscura. In this species, both copies of the gene are transcribed and, like in D. melangaster, only expressed in males. The duplicated region includes 300 bp upstream of the gene that would therefore seem sufficient for their expression in males. This region presents at its 5' end a stretch 93-bp that has a high similarity with the corresponding region of D. melanogaster and could be part of a still unidentified regulatory element of these genes.
克隆并测序了果蝇（Drosphila subobscura）中与黑腹果蝇（D. melanogaster）的Acp70A区域同源的一个3.1千碱基对区域，该区域包含性肽基因。该区域包含一个约600碱基对的重复序列，其中包括性肽及其5'和3'侧翼区域。前体蛋白分别长54和56个氨基酸（相比之下，黑腹果蝇中为55个氨基酸），并且每个都包含一个19个氨基酸长的信号肽。成熟肽的C末端部分在黑腹果蝇和果蝇的两个拷贝之间高度保守。在这个物种中，该基因的两个拷贝都被转录，并且与黑腹果蝇一样，仅在雄性中表达。重复区域包括该基因上游300碱基对，因此似乎足以使其在雄性中表达。该区域在其5'端有一段93碱基对的序列，与黑腹果蝇的相应区域高度相似，可能是这些基因中尚未确定的调控元件的一部分。
