Schmidt T, Choffat Y, Schneider M, Hunziker P, Fuyama Y, Kubli E
Zoological Institute, University Zurich-Irchel, Switzerland.
Insect Biochem Mol Biol. 1993 Jul;23(5):571-9. doi: 10.1016/0965-1748(93)90030-v.
A peptide homologous to the Drosophila melanogaster sex-peptide (SP) was isolated from Drosophila suzukii accessory glands and its amino acid sequence determined. The D. suzukii peptide contains 41 amino acids and has a calculated molecular weight of 5100 Da. Comparison of the sequences reveals strong homologies in the N-terminal and C-terminal parts of the peptides. In the D. suzukii sex-peptide, however, five additional amino acids are inserted after amino acid 7. Based on the sequence of the peptide, a cDNA coding for the D. suzukii peptide was isolated by PCR. Sequence analysis of the cDNA confirmed the SP amino acid sequence determined by peptide sequencing. Furthermore, based on the cDNA sequence, we isolated the D. suzukii sex-peptide gene by inverse PCR. The D. suzukii sex-peptide gene contains an intron and codes for a 60 amino acid precursor. The D. melanogaster and the D. suzuki sex-peptides elicit rejection behaviour in the presence of males and an increased egg laying in virgin females of both species.
从铃木果蝇的附腺中分离出一种与黑腹果蝇性肽（SP）同源的肽，并确定了其氨基酸序列。铃木果蝇肽含有41个氨基酸，计算分子量为5100 Da。序列比较显示，这些肽在N端和C端部分具有很强的同源性。然而，在铃木果蝇性肽中，在第7个氨基酸之后插入了另外5个氨基酸。根据该肽的序列，通过PCR分离出了编码铃木果蝇肽的cDNA。cDNA的序列分析证实了通过肽测序确定的SP氨基酸序列。此外，基于cDNA序列，我们通过反向PCR分离出了铃木果蝇性肽基因。铃木果蝇性肽基因含有一个内含子，编码一个60个氨基酸的前体。黑腹果蝇和铃木果蝇的性肽在有雄性存在时会引发排斥行为，并且会使这两个物种的未交配雌性产卵增加。
