McHugh T D, Gillespie S H
Department of Microbiology, Royal Free Hospital School of Medicine, London, United Kingdom.
J Clin Microbiol. 1998 May;36(5):1410-3. doi: 10.1128/JCM.36.5.1410-1413.1998.
IS6110 restriction fragment length polymorphism typing is now established as the primary typing method for Mycobacterium tuberculosis. It has been assumed that the position of bands is random. Thus, the discrimination of the technique increases in proportion to the copy number. Two collections of M. tuberculosis were investigated to test this hypothesis. We identified 33 positions in isolates from a Tanzanian collection and 25 positions in isolates from a London, United Kingdom, collection where bands were significantly more likely to be present than would be expected by chance. These data suggest that band position is not random, and this possibility may have an impact on the interpretation of molecular epidemiological studies of M. tuberculosis.
IS6110 限制性片段长度多态性分型现已成为结核分枝杆菌的主要分型方法。一直以来都认为条带的位置是随机的。因此，该技术的鉴别能力随拷贝数成比例增加。对两组结核分枝杆菌样本进行了研究以验证这一假设。我们在来自坦桑尼亚的一组分离株中确定了33个条带位置，在来自英国伦敦的一组分离株中确定了25个条带位置，在这些位置出现条带的可能性明显高于随机预期。这些数据表明条带位置并非随机，这种可能性可能会对结核分枝杆菌分子流行病学研究的解读产生影响。
