Dale Jeremy W, Al-Ghusein Hasan, Al-Hashmi Salim, Butcher Philip, Dickens Anne L, Drobniewski Francis, Forbes Ken J, Gillespie Stephen H, Lamprecht Dianie, McHugh Timothy D, Pitman Richard, Rastogi Nalin, Smith Andrew T, Sola Christophe, Yesilkaya Hasan
School of Biomedical and Life Sciences, University of Surrey, Guildford, Surrey GU2 7XH, United Kingdom.
J Bacteriol. 2003 Apr;185(8):2555-62. doi: 10.1128/JB.185.8.2555-2562.2003.
Molecular typing of Mycobacterium tuberculosis by using IS6110 shows low discrimination when there are fewer than five copies of the insertion sequence. Using a collection of such isolates from a study of the epidemiology of tuberculosis in London, we have shown a substantial degree of congruence between IS6110 patterns and both spoligotype and PGRS type. This indicates that the IS6110 types mainly represent distinct families of strains rather than arising through the convergent insertion of IS6110 into favored positions. This is supported by identification of the genomic sites of the insertion of IS6110 in these strains. The combined data enable identification of the putative evolutionary relationships of these strains, comprising three lineages broadly associated with patients born in South Asia (India and Pakistan), Africa, and Europe, respectively. These lineages appear to be quite distinct from M. tuberculosis isolates with multiple copies of IS6110.
当插入序列少于5个拷贝时，使用IS6110对结核分枝杆菌进行分子分型的鉴别力较低。通过对伦敦结核病流行病学研究中收集的此类分离株进行分析，我们发现IS6110分型与 spoligotype分型和PGRS分型之间存在高度一致性。这表明IS6110类型主要代表不同的菌株家族，而非通过IS6110在优势位置的趋同插入产生。对这些菌株中IS6110插入的基因组位点进行鉴定也支持了这一点。综合数据能够确定这些菌株的假定进化关系，它们分别包括与出生在南亚（印度和巴基斯坦）、非洲和欧洲的患者广泛相关的三个谱系。这些谱系似乎与具有多个IS6110拷贝的结核分枝杆菌分离株截然不同。
