Hartas J, Hibble M, Sriprakash K S
Menzies School of Health Research, Darwin, Causarina NT, Australia.
J Clin Microbiol. 1998 May;36(5):1428-9. doi: 10.1128/JCM.36.5.1428-1429.1998.
We describe a simplification of a highly discriminatory molecular typing method, called Vir typing, for Streptococcus pyogenes (D. Gardiner, J. Hartas, B. Currie, J. D. Mathews, D. J. Kemp, and K. S. Sriprakash, PCR Methods Appl. 4:288-293, 1995). The procedure can be completed within a day, is reproducible, and can be applied directly to colonies growing on primary culture plates, allowing rapid establishment of strain identity in an outbreak.
我们描述了一种高度 discriminatory 的分子分型方法(称为 Vir 分型)的简化版,用于化脓性链球菌(D. Gardiner、J. Hartas、B. Currie、J. D. Mathews、D. J. Kemp 和 K. S. Sriprakash,《PCR 方法应用》4:288 - 293,1995 年)。该程序可在一天内完成,具有可重复性,并且可以直接应用于在原代培养平板上生长的菌落,从而在疫情爆发时快速确定菌株身份。