Osterlund A, Engstrand L
Department of Clinical Microbiology, University Hospital, Uppsala, Sweden.
Scand J Infect Dis. 1995;27(2):119-21. doi: 10.3109/00365549509018990.
In recent years, several DNA fingerprinting methods have been tried for the epidemiological investigation of Streptococcus pyogenes isolates. We chose the Random Amplified Polymorphic DNA (RAPD) method for the typing of pre- and post-antibiotic treatment S. pyogenes isolates obtained from 14 patients with pharyngotonsillitis. Isolates from 4 patients had been collected 10 years earlier than from the others. Hence epidemiological connections between the strains were unlikely. Both pre- and post-antibiotic treatment isolates showed identical RAPD patterns, indicating that recurrences in these patients had been caused by homologous strains of S. pyogenes. It was possible by means of RAPD to differentiate between 12 different T-types and occasionally between clones of the same serotype of S. pyogenes. The RAPD method is an easier alternative to traditional DNA-typing techniques and provides quick results with minimal technical complexity.
近年来，已尝试多种DNA指纹图谱方法用于化脓性链球菌分离株的流行病学调查。我们选择随机扩增多态性DNA（RAPD）方法对从14例咽扁桃体炎患者中获得的抗生素治疗前后的化脓性链球菌分离株进行分型。4例患者的分离株比其他患者的分离株早10年收集。因此，这些菌株之间的流行病学联系不太可能存在。抗生素治疗前后的分离株均显示出相同的RAPD模式，表明这些患者的复发是由化脓性链球菌的同源菌株引起的。通过RAPD可以区分12种不同的T型，偶尔也可以区分化脓性链球菌同一血清型的克隆。RAPD方法是传统DNA分型技术的一种更简便的替代方法，以最小的技术复杂性提供快速结果。
