McNally K J, Peters A
Department of Anatomy and Neurobiology, Boston University School of Medicine, Boston, Massachusetts, USA.
J Histochem Cytochem. 1998 Apr;46(4):541-5. doi: 10.1177/002215549804600415.
We describe a new method for intense staining of myelin. The stain involves immersing frozen or vibratome sections of 4% normal horse serum. A DAB reaction is then carried out, which results in the deposition of reaction product in myelin sheaths. On intensification of this reaction product using the silver enhancement technique described by Görcs, myelin stains an intense black color, making the preparations suitable for photography. The stain is especially useful for determining the distribution of myelinated fibers in gray matter.
我们描述了一种用于髓鞘强烈染色的新方法。该染色方法包括将切片浸入4%正常马血清中进行冷冻或振动切片。然后进行DAB反应，这会导致反应产物沉积在髓鞘中。使用Görcs描述的银增强技术增强该反应产物后，髓鞘会染成深黑色，使标本适合拍照。该染色方法对于确定灰质中有髓纤维的分布特别有用。
