Carriel Víctor, Campos Antonio, Alaminos Miguel, Raimondo Stefania, Geuna Stefano
Tissue Engineering Group, Department of Histology, Faculty of Medicine, University of Granada, Avenida de la investigación n° 11, Torre A, 5ª planta, 18016, Granada, Spain.
Instituto de Investigación Biosanitaria Ibs, Granada, Spain.
Methods Mol Biol. 2017;1560:207-218. doi: 10.1007/978-1-4939-6788-9_15.
Histochemical techniques enable the specific identification of myelin by light microscopy. Here we describe three histochemical methods for the staining of myelin suitable for formalin-fixed and paraffin-embedded materials. The first method is conventional luxol fast blue (LFB) method which stains myelin in blue and Nissl bodies and mast cells in purple. The second method is a LBF-based method called MCOLL, which specifically stains the myelin as well the collagen fibers and cells, giving an integrated overview of the histology and myelin content of the tissue. Finally, we describe the osmium tetroxide method, which consist in the osmication of previously fixed tissues. Osmication is performed prior the embedding of tissues in paraffin giving a permanent positive reaction for myelin as well as other lipids present in the tissue.
组织化学技术能够通过光学显微镜对髓磷脂进行特异性鉴定。在此，我们描述三种适用于福尔马林固定和石蜡包埋材料的髓磷脂染色组织化学方法。第一种方法是传统的卢戈氏固绿（LFB）法，该方法将髓磷脂染成蓝色，将尼氏体和肥大细胞染成紫色。第二种方法是基于LBF的称为MCOLL的方法，它能特异性地对髓磷脂以及胶原纤维和细胞进行染色，从而对组织的组织学和髓磷脂含量给出全面的概述。最后，我们描述四氧化锇法，该方法包括对先前固定的组织进行锇化处理。锇化处理在组织石蜡包埋之前进行，可使髓磷脂以及组织中存在的其他脂质产生永久性阳性反应。
