Komagata Y, Komiyama K, Nomura S
Bio-Iatoric Center, Kitasato Institute, Tokyo.
Jpn J Antibiot. 1998 Feb;51(2):130-6.
Antibacterial activity of clindamycin against Propionibacterium acnes (P. acnes) was evaluated in comparison with nadifloxacin in vitro. Using a burned-infected mouse model, topical application of 1% gel form of clindamycin phosphate on P. acnes was also evaluated in in vivo. (1) The MIC of clindamycin measured by agar dilution method was 0.02 microgram/ml, and this value was smaller than that of nadifloxacin (0.3 microgram/ml). (2) At concentrations on 1-, 2- and 4- times the MIC clindamycin demonstrated bacteriostatic activity on P. acnes and showed bactericidal activity at 5-times the MIC. Nadifloxacin showed bacteriostatic activity at one half the MIC and bactericidal activity at the MIC. (3) Against acquired resistant strains of P. acnes, the highest concentrations of clindamycin and nadifloxacin that did not inhibit growth of the organism increased 5-fold higher than those against sensitive strain during 25 successive cultures in vitro. Therefore, the resistance of P. acnes was found to be emerged at almost the same ratio against both agents. (4) The chemotherapeutic effects of 1% gel form of clindamycin phosphate and 1% cream of nadifloxacin were evaluated for given subcutaneously to infected P. acnes at the burned site in mice. The topical application of either agents showed a significant reduction of number of bacteria and this result predicted clinical efficacy of topical application of clindamycin.
在体外，将克林霉素对痤疮丙酸杆菌的抗菌活性与那氟沙星进行了比较评估。使用烧伤感染小鼠模型，还在体内评估了1%克林霉素磷酸酯凝胶剂对痤疮丙酸杆菌的局部应用效果。（1）通过琼脂稀释法测得克林霉素的最低抑菌浓度（MIC）为0.02微克/毫升，该值低于那氟沙星（0.3微克/毫升）。（2）在MIC的1倍、2倍和4倍浓度下，克林霉素对痤疮丙酸杆菌表现出抑菌活性，在MIC的5倍浓度时表现出杀菌活性。那氟沙星在MIC的一半浓度时表现出抑菌活性，在MIC时表现出杀菌活性。（3）对于痤疮丙酸杆菌的获得性耐药菌株，在体外连续传代培养25次的过程中，克林霉素和那氟沙星不抑制该菌生长的最高浓度比针对敏感菌株时增加了5倍。因此，发现痤疮丙酸杆菌对这两种药物产生耐药的比例几乎相同。（4）评估了1%克林霉素磷酸酯凝胶剂和1%那氟沙星乳膏对小鼠烧伤部位皮下接种痤疮丙酸杆菌后的化疗效果。两种药物的局部应用均显示细菌数量显著减少，这一结果预示了克林霉素局部应用的临床疗效。
