[脂血血浆对血液凝固试验的干扰。采用正己烷清除法进行校正]
[Interference in blood coagulation tests on lipemic plasma. Correction using n-hexane clearing].
作者信息
Arambarri M, Oriol A, Sancho J M, Roncalés F J, Galán A, Galimany R
机构信息
Servicio de Análisis Clínicos, Hospital Universitari Germans Trias i Pujol, Universidad Autónoma de Barcelona.
出版信息
Sangre (Barc). 1998 Feb;43(1):13-9.
PURPOSE
To eliminate the bias in the plasmatic coagulation test caused by lipemic samples by means of with addition of n-hexane in the ACL-3000 analyzer (Instrumentation Laboratory).
MATERIAL AND METHODS
The turbidity caused by lipemia was simulated by addition of increasing quantities of Intralipid 20% (interferent) to samples from patients with normal pathological coagulation tests (with oral anticoagulant therapy). We compared the results of PT (prothrombin time), fibrinogen (derived) and APTT (activated partial thromboplastin time) before and after the addition of n-hexane.
RESULTS
In samples with an elevated concentration of interferent (> 9.79 mmol/L of triglycerides) the analyzer failed to give any results. Once the lipids were cleared by n-hexane, the analyzer offered results for all the samples, which coincided with the original sample after applying a correction factor 1.06 to the TP and 0.88 to the TTPA.
CONCLUSION
The addition of n-hexane is a valid method to eliminate the bias caused by the lipids in the coagulation tests.
目的
通过在ACL - 3000分析仪（仪器实验室）中添加正己烷，消除脂血样本对血浆凝血试验造成的偏差。
材料与方法
通过向凝血功能正常（接受口服抗凝治疗）患者的样本中添加越来越多的20%英脱利匹特（干扰物）来模拟脂血引起的浊度。我们比较了添加正己烷前后凝血酶原时间（PT）、纤维蛋白原（衍生）和活化部分凝血活酶时间（APTT）的结果。
结果
在干扰物浓度升高（甘油三酯>9.79 mmol/L）的样本中，分析仪无法给出任何结果。一旦用正己烷清除脂质，分析仪就能为所有样本提供结果，在对凝血酶时间（TP）应用校正因子1.06、对活化部分凝血活酶时间（TTPA）应用校正因子0.88后，这些结果与原始样本一致。
结论
添加正己烷是消除凝血试验中脂质引起的偏差的有效方法。
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