Smith G E, Anderson E C, Burridge M J, Peter T F, Mahan S M
University of Florida/USAID/SADC Heartwater Research Project, Veterinary Research Laboratory, Causeway, Harare, Zimbabwe.
J Wildl Dis. 1998 Apr;34(2):297-304. doi: 10.7589/0090-3558-34.2.297.
Endothelial cell cultures were established from several wild African mammalian species. Long-term cultures were established from three ruminants, stable antelope (Hippotragus niger), buffalo (Syncerus caffer), and eland (Tragelaphus oryx), and from an omnivore, the bushpig (Potamochoerus porcus). Cowdria ruminanntium was isolated from plasma of clinically affected animals in these four cell lines and in bovine endothelial cells used routinely for C. ruminantium propagation. Nineteen different strains of C. ruminantium from Africa and the Caribbean region were grown and maintained in these cell lines and their growth was comparable with growth in the bovine endothelial cells. The role of sable antelope, eland, and bushpigs in the epidemiology of heartwater is unknown. However, these results extend the number of cell lines that can be used to isolate and grow C. ruminantium.
从几种野生非洲哺乳动物物种中建立了内皮细胞培养物。从三种反刍动物，即南非大羚羊（黑马羚）、水牛（非洲水牛）和大羚羊，以及一种杂食动物，即非洲野猪中建立了长期培养物。在这四种细胞系以及常规用于反刍兽立克次氏体繁殖的牛内皮细胞中，从临床患病动物的血浆中分离出了反刍兽立克次氏体。来自非洲和加勒比地区的19种不同的反刍兽立克次氏体菌株在这些细胞系中生长和维持，它们的生长情况与在牛内皮细胞中的生长情况相当。南非大羚羊、大羚羊和非洲野猪在心水病流行病学中的作用尚不清楚。然而，这些结果增加了可用于分离和培养反刍兽立克次氏体的细胞系数量。
