Borchman D, Yappert M C
Department of Ophthalmology and Visual Sciences, University of Louisville, Kentucky 40202, USA.
Invest Ophthalmol Vis Sci. 1998 May;39(6):1053-8.
To quantify age-related changes in products of lipid oxidation in human lenses and to relate these changes to membrane hydrocarbon chain structure. Deviation from a well-defined membrane-lipid composition and structure could result in alterations in membrane function and disruption of the homeostasis of the cell.
Infrared spectroscopy was used to detect lipid compositional and structural changes in human lens membranes associated with age and cataracts.
Lipid oxidation increased linearly threefold relative to total phospholipids in subjects ranging in age between 1 and 85 years, as was evident by increases in trans double bonds, lipid carbonyls, and secondary products. There was no statistical difference between the levels of lipid oxidation in the cortex or nucleus. Lipid hydrocarbon chain order (rigidity) increased from approximately 40% at birth to 70% at 80 years of age. Changes in lipid order correlated with changes in the relative content of membrane phosphatidylcholine and sphingomyelin, and with the level of lipid oxidation.
Lipid oxidation increased linearly and uniformly throughout the human lens with age. The change in lipid oxidation with age correlated to a change in lipid order.
量化人晶状体中脂质氧化产物随年龄的变化，并将这些变化与膜烃链结构相关联。偏离明确界定的膜脂质组成和结构可能导致膜功能改变以及细胞内稳态的破坏。
使用红外光谱法检测与年龄和白内障相关的人晶状体膜中脂质组成和结构的变化。
在1至85岁的受试者中，相对于总磷脂，脂质氧化线性增加了三倍，这从反式双键、脂质羰基和次级产物的增加中明显可见。皮质或核中的脂质氧化水平之间没有统计学差异。脂质烃链有序度（刚性）从出生时的约40%增加到80岁时的70%。脂质有序度的变化与膜磷脂酰胆碱和鞘磷脂的相对含量变化以及脂质氧化水平相关。
随着年龄的增长，人晶状体中脂质氧化线性且均匀地增加。脂质氧化随年龄的变化与脂质有序度的变化相关。
