Xu L, Glassford A J, Giaccia A J, Giffard R G
Department of Anesthesia, Stanford University School of Medicine, CA 94305, USA.
Neuroreport. 1998 Mar 30;9(5):875-9. doi: 10.1097/00001756-199803300-00021.
Acidosis is a well established concomitant of tissue ischemia. Acidosis in the pH range 6.0-7.0 is seen in cerebral ischemia and within solid tumors. Extracellular acidosis of pH 6.0 and 6.4 provided essentially complete protection from 48 h serum deprivation induced apoptotic death of cultured primary murine neurons. We tested the effect of p53 using transformed mouse embryo fibroblasts of either p53+/+ or p53-/- genotype. Both were markedly protected from serum deprivation by acidity. Hypoxia induced fibroblast injury was also reduced at pH 6.8. Lower pH resulted in a shift from apoptotic to necrotic morphology after 42 h hypoxia. Acidosis reduces apoptosis of both normal and transformed cells, irrespective of p53 status.
酸中毒是组织缺血的一种公认的伴随症状。在脑缺血和实体肿瘤中可观察到pH值范围为6.0 - 7.0的酸中毒。pH值为6.0和6.4的细胞外酸中毒能为培养的原代小鼠神经元提供基本完全的保护，使其免受48小时血清剥夺诱导的凋亡死亡。我们使用p53 +/+ 或p53 -/- 基因型的转化小鼠胚胎成纤维细胞测试了p53的作用。二者均因酸度而显著免受血清剥夺的影响。在pH 6.8时，缺氧诱导的成纤维细胞损伤也有所减少。较低的pH值导致在42小时缺氧后细胞形态从凋亡转变为坏死。酸中毒可减少正常细胞和转化细胞的凋亡，与p53状态无关。
