Hedley S J, Gawkrodger D J, Weetman A P, Morandini R, Boeynaems J M, Ghanem G, Neil S M
University Department of Medicine, Clinical Sciences Centre, Northern General Hospital, Sheffield, U.K.
Br J Dermatol. 1998 Mar;138(3):536-43. doi: 10.1046/j.1365-2133.1998.02209.x.
alpha-Melanocyte stimulating hormone (alpha-MSH) was found significantly to reduce tumour necrosis factor-alpha (TNF-alpha) stimulated upregulation of intercellular adhesion molecule-1 (ICAM-1) in normal adult cutaneous melanocytes. The maximum inhibitory response to alpha-MSH was obtained at around 10(-10) mol/L alpha-MSH when cells were coincubated with alpha-MSH and TNF-alpha for 24 h. alpha-MSH had little or no effect on basal ICAM-1 expression in melanocytes and the effects of alpha-MSH could be mimicked with 3-isobutyl-1-methylxanthine (IBMX). Preliminary data in three human melanoma cell lines also showed alpha-MSH and forskolin to be effective in significantly reducing TNF-alpha stimulated ICAM-1 expression over 24 h. The extent of the inhibition varied from cell line to cell line and was greatest in those cells with the highest number of alpha-MSH receptors. These data suggest that alpha-MSH has the ability to oppose the action of the pro-inflammatory cytokine TNF-alpha on melanocytes and melanoma cells.
α-黑素细胞刺激素（α-MSH）被发现可显著降低肿瘤坏死因子-α（TNF-α）刺激的正常成人皮肤黑素细胞中细胞间黏附分子-1（ICAM-1）的上调。当细胞与α-MSH和TNF-α共同孵育24小时时，在约10⁻¹⁰mol/L的α-MSH浓度下可获得对α-MSH的最大抑制反应。α-MSH对黑素细胞中ICAM-1的基础表达几乎没有影响，并且α-MSH的作用可以被3-异丁基-1-甲基黄嘌呤（IBMX）模拟。在三种人黑色素瘤细胞系中的初步数据也表明，α-MSH和福斯可林在24小时内可有效显著降低TNF-α刺激的ICAM-1表达。抑制程度因细胞系而异，在具有最高数量α-MSH受体的细胞中最大。这些数据表明，α-MSH有能力对抗促炎细胞因子TNF-α对黑素细胞和黑色素瘤细胞的作用。
