关于诺加霉素对哺乳动物组织学、细胞学和细胞遗传学影响的进一步研究。

Further studies on the histological cytological and cytogenetic effects of nogalamycin in mammals.

作者信息

Bempong M A, Butts R M

出版信息

Mutat Res. 1976 Jul;40(3):251-60. doi: 10.1016/0165-1218(76)90051-3.

Abstract

The histological, cytological and cytogenetic effects of nogalamycin were studied in rats, mice and cultured human leukocytes. Four standard test systems were used in the cytological studies: (1)analysis of spermatogonial cells, (2)cytological evaluation of bone marrow metaphase plates, (3)micronucleus test in polychromatic erythrocytes, and (4)determination of chromosomal aberrations in cultured human leukocytes. The results of the studies indicated that the types and frequenices of cytological errors induced by the drug were not significantly different from the concurrent control. The reduced incidence of fertile matings from 22 to 35 days after treatment is attributed to the cytostatic effect of nogalamycin (NM) on spermatogonia and spermatocytes. Subsidence of NM-induced inhibition of spermatogonium differentiation and spermatocyte maturation occurred 37 days post-treatment. Accordingly, fertile matings, which had reached a zero level by 23rd day, occurred 35 days after treatment, and reached the control level between 47 and 49 days post-treatment.

摘要

对诺加霉素在大鼠、小鼠及培养的人白细胞中的组织学、细胞学及细胞遗传学效应进行了研究。在细胞学研究中使用了四种标准测试系统：(1)精原细胞分析；(2)骨髓中期板的细胞学评估；(3)多染性红细胞微核试验；(4)培养的人白细胞中染色体畸变的测定。研究结果表明，该药物诱导的细胞学错误类型和频率与同期对照无显著差异。治疗后22至35天可育交配发生率降低归因于诺加霉素（NM）对精原细胞和精母细胞的细胞生长抑制作用。NM诱导的精原细胞分化和精母细胞成熟抑制在治疗后37天消退。因此，在治疗后35天出现了可育交配，到第23天时可育交配率已降至零水平，在治疗后47至49天达到对照水平。