葡萄牙沙眼衣原体泌尿生殖道分离株的基因分型
Genotyping of Portuguese Chlamydia trachomatis urogenital isolates.
作者信息
Borrego M J, Gomes J P, Lefebvre J F, Eb F, Orfila J, Catry M A
机构信息
Laboratório de Bacteriologia, Instituto Nacional de Saúde, Lisboa, Portugal.
出版信息
Genitourin Med. 1997 Dec;73(6):561-3. doi: 10.1136/sti.73.6.561.
OBJECTIVE
To determine the prevalence of the different Chlamydia trachomatis genotypes in Portuguese patients.
METHODS
Urogenital isolates (n = 240) derived from attenders of various clinics in the Lisbon area were differentiated into genovars by genotyping with restriction fragment length polymorphism (RFLP) analysis of the PCR amplified omp1 gene.
RESULTS
Genotype E was the most common for both men (47.9%) and women (43.8%). Genotypes D and F were the second most prevalent for men (11.3%) and genotype H was the second most prevalent for women (19.5%). Genotypes F, G, D, in women and H, G, I, in men, were found in a lower percentage of cases. Genotypes B, Ba, J, K, L1 and L2 were very rarely detected.
CONCLUSIONS
With one exception, the overall distribution of Chlamydia trachomatis genotypes in our study is similar to what has been observed in other western countries. The only exception is the unusual prevalence of genotype H among women. The clinical manifestations associated with this and other genotypes were similar.
目的
确定葡萄牙患者中不同沙眼衣原体基因型的流行情况。
方法
通过对聚合酶链反应（PCR）扩增的omp1基因进行限制性片段长度多态性（RFLP）分析进行基因分型，将来自里斯本地区各诊所就诊者的240份泌尿生殖道分离株分为不同的基因变种。
结果
基因型E在男性（47.9%）和女性（43.8%）中最为常见。基因型D和F在男性中位列第二常见（11.3%），基因型H在女性中位列第二常见（19.5%）。基因型F、G、D在女性中以及基因型H、G、I在男性中所占比例较低。基因型B、Ba、J、K、L1和L2极少被检测到。
结论
除一个例外情况外，我们研究中沙眼衣原体基因型的总体分布与其他西方国家观察到的情况相似。唯一的例外是基因型H在女性中异常流行。与该基因型及其他基因型相关的临床表现相似。
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