Lan J, Ossewaarde J M, Walboomers J M, Meijer C J, van den Brule A J
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
J Clin Microbiol. 1994 Feb;32(2):528-30. doi: 10.1128/jcm.32.2.528-530.1994.
Successful amplification of omp1 DNA by PCR is crucial in the genotyping of Chlamydia trachomatis when directly performed with clinical samples (J. Lan, J. M. M. Walboomers, R. Roosendaal, G. J. van Doornum, D. M. McLaren, C. J. L. M. Meijer, and A. J. C. van den Brule, J. Clin. Microbiol. 31:1060-1065, 1993). Several primers flanking the four variable domains of the omp1 gene were selected and tested for sensitivity in several nested PCRs with serial dilutions of serovar G. The optimal sensitivity obtained was 0.1 to 0.01 inclusion-forming units, similar to that obtained in the C. trachomatis plasmid PCR. With this approach, any C. trachomatis PCR-positive sample can be typed.
当直接对临床样本进行检测时,通过聚合酶链反应(PCR)成功扩增沙眼衣原体omp1基因的DNA对于该病原体的基因分型至关重要(J. Lan、J. M. M. Walboomers、R. Roosendaal、G. J. van Doornum、D. M. McLaren、C. J. L. M. Meijer和A. J. C. van den Brule,《临床微生物学杂志》31:1060 - 1065,1993年)。选择了几个位于omp1基因四个可变区侧翼的引物,并在对血清型G进行系列稀释的几个巢式PCR中测试其敏感性。获得的最佳敏感性为0.1至0.01个包涵体形成单位,与沙眼衣原体质粒PCR中获得的敏感性相似。采用这种方法,任何沙眼衣原体PCR阳性样本都可以进行分型。