Kaluniants K A, Strel'nikova L I, Shteĭn I V
Prikl Biokhim Mikrobiol. 1979 Jan-Feb;15(1):57-62.
The paper gives data on the influence of different sources of carbon, nitrogen and phosphorus on the accumulation of biomass and synthesis of neutral protease by Bacillus subtilis str. 103. The highest proteolytic activity was obtained on the medium containing maltose or hydrolyzed starch as a carbon source, monopotassium phosphate as a phosphorus source, and ammonium sulphate as a nitrogen source. The enzyme activity increased upon an addition of albumin, peptone or casein. A study of the amino acid effect showed that methionine, isoleucine and valine stimulated the protease synthesis to the greatest extent.
该论文给出了不同碳源、氮源和磷源对枯草芽孢杆菌103菌株生物量积累和中性蛋白酶合成影响的数据。在以麦芽糖或水解淀粉作为碳源、磷酸二氢钾作为磷源、硫酸铵作为氮源的培养基上，获得了最高的蛋白水解活性。添加白蛋白、蛋白胨或酪蛋白后，酶活性增强。氨基酸效应研究表明，蛋氨酸、异亮氨酸和缬氨酸对蛋白酶合成的刺激作用最大。
