[辐射后大鼠胸腺和脾淋巴细胞中染色质的核酸内切酶解作用]
[Endonucleolysis of chromatin in rat thymus and spleen lymphocytes after irradiation].
作者信息
Solodushko V O, Matyshevs'ka O P, Kucherenko N Ie
机构信息
Taras Shevchenko State University, Kyiv, Ukraine.
出版信息
Ukr Biokhim Zh (1978). 1997 Jul-Aug;69(4):88-94.
The level of soluble DNA fragments (polydesoxynucleotides) in thymus and spleen lymphocytes is enhanced 12 h after the whole-body X-ray irradiation in dose of 0.5 and 1 Gy. The level of Ca2+, Mg(2+)-DNAase activity in lysed cells extracts, measured with calf spleen DNA, was enhanced in the region of 0.1-0.5 mM CaCl2 in incubation medium and decreased at 5 mM CaCl2. Monitoring of DNA fragmentation by electrophoresis in agarose gels showed the presence of fragments with approximately 200, 400, 800 bp length. X-ray induced DNA fragmentation is supposed to be associated with Ca2+, Mg(2+)-endonuclease activation.
在接受0.5戈瑞和1戈瑞全身X射线照射12小时后，胸腺和脾脏淋巴细胞中可溶性DNA片段（多脱氧核苷酸）的水平升高。用小牛脾脏DNA测定的裂解细胞提取物中Ca2 +、Mg(2 +)-DNA酶活性，在孵育培养基中0.1 - 0.5 mM CaCl2区域增强，在5 mM CaCl2时降低。通过琼脂糖凝胶电泳监测DNA片段化显示存在长度约为200、400、800碱基对的片段。X射线诱导的DNA片段化被认为与Ca2 +、Mg(2 +)-核酸内切酶激活有关。
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